Biomaterials 30, 6621-6628. Tested in Western Blot (WB) and Immunohistochemistry (Paraffin) (IHC (P)) applications. Bone Sialoprotein. The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. BSP is strongly expressed in bone and we previously showed that BSP knockout (BSP-/-) mice have a higher bone mass than wild type (BSP+/+) littermates, with lower bone remodelling. The gene for bone sialoprotein or integrin-binding sialoprotein (IBSP) is located on chromosome 4q21-q25, which is also the location of the distinct SPP1 gene for osteopontin. It has a putative N-terminal signal sequence, followed by a tyrosine-rich repeat, 3 acidic clusters, and 2 C-terminal tyrosine-rich repeats separated by an RGD motif. Immunostaining confirmed the loss of Bsp protein in 1D11-treated lung metastases, and TGF- was shown to regulate and correlate with Bsp expression in vitro. Results demonstrated that PTHrP repressed gene expression and protein synthesis of bone sialoprotein (BSP) and abolished cementoblast-mediated biomineralization in vitro. Accumulating evidence supports BSP as a key regulator of mineralized tissue formation via evolutionarily conserved functional domains, including a C-terminal integrin-binding Arg-Gly-Asp (RGD) domain implicated in extracellular matrix-cell signaling. Bone sialoprotein (Bsp) is a member of the SIBLING gene family. . It belongs to the Small Integrin Binding Ligand N-linked Glycoprotein (SIBLING) gene family 30. Therefore, regulation of the bone sialoprotein gene is potentially important in the differentiation of osteoblasts, bone matrix mineralization and tumor metastasis. Bone sialoprotein (BSP) is a noncollagenous matrix glycoprotein localized predominantly in mineralized tissues but also detected in extraskeletal sites undergoing focal mineralization. BSP is also known as IBSP (integrin-binding sialoprotein). This antibody reacts with Mouse, Rat samples. 1-4 Hydrogels have been mainly considered for soft tissue regeneration, 5 but recent advances have allowed for the creation of multifunctional biomaterials with applications to hard . IDs. In this study, we examined the ability of EMD to regulate BSP gene transcription in osteoblast-like cells. Bone sialoprotein II is one of the major noncollagenous proteins in the extracellular matrix of bone. Osteocalcin (5-6 kDa) and bone sialoprotein (72-80 kDa) are synthesized and secreted by normal maturing osteoblasts. Treatment of osteoblast-like ROS 17/2.8 cells with LPS (1 g/ml) for 12 h caused a marked reduction in BSP mRNA levels. . Although the RUNX2 transcription factor directly regulates many osteoblast-related genes, its function in Bsp expression remains uncertain. Introduction. The proximal promoter is highly conserved in mammals and is bound by runt related transcription factor 2, cAMP responsive element binding protein 1, and AP-1 family transcription factors. For western blotting, BSP antibody (bone sialoprotein; MAB1061, Millipore, Burlington, USA), COL I antibody (collagen type I, MAB3391, Millipore), and OCN antibody (osteocalcin, sc-74495, Santa Cruz, USA) were used. The protein has been found in osteoblasts and osteocytes. 7.1. . The protein encoded by this gene is a major structural protein of the bone matrix. (2018 Apr): 240-255. 10.1016/j.biomaterials.2009.08.032 [Google Scholar] Chambers H. F., Deleo F. R. (2007). gene page . Background of IBSP/Sialoprotein II. (2008) by Y Ogata Venue: J Periodontal Res: Add To MetaCart. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. The protein encoded by this gene is a major structural protein of the bone matrix. They are major noncollagenous bone matrix proteins, with osteocalcin comprising 1% to 2% of the total proteins in the skeleton .Osteocalcin binds with high affinity to hydroxyapatite crystals, the key mineral component of bone, and regulates bone crystal growth . Bone sialoprotein (BSP), an early phenotypic marker of osteoblast differentiation, has been implicated in the nucleation of hydroxyapatite during bone formation. Gene expression is more limited than that for osteopontin [243 ]. It constitutes approximately 12% of the . Bone Sialoprotein (IBSP) (native) Rabbit Polyclonal Antibody CAT#: AP02056SU-N Write a review Bone Sialoprotein (IBSP) (native) rabbit polyclonal antibody, Serum Datasheet SDS USD 651.00 Availability* 2 Weeks Size 100 ul Request Bulk or Custom Service Specifications Documents Resources Other products for "IBSP" cDNA Clones Lentiviral CRISPR RNAi . Treatment of osteoblastlike ROS 17/2.8 cells with LPS (1 g/ml) for 12 h caused a marked reduction in BSP mRNA levels. The intron/exon junctions defined by sequence analysis are of class 0, retaining an intact coding triplet. Gene silencing of OPN reduced the proliferation, adhesion and migration of another human colon carcinoma, LoVo cells. This record represents the promoter and related 5' regulatory region of the integrin binding sialoprotein gene, also known as BSP. We have previously characterized the human BSP gene and have shown that the upstream sequence contains inverted TATA and CCAAT motifs at the expected locations from the transcriptional start site (J. M. Kerr et . Runx2 has an essential role in the maturation of osteoblasts by binding to its target promoters and enhancers of various other bone-specific target genes, including collagen type-I, osteopontin, and bone sialoprotein through its runt homology domain. The encoded protein is secreted and binds hydroxyapatite with high affinity. Bone sialoprotein (BSP) is a mineralized tissue-specific protein that appears to function in the initial mineralization of bone. WVID1(9C5) mouse bone sialoprotein II antibody To provide a better shopping experience, our website uses cookies. Their genes are grouped as a "bone gene cluster" on human chromosome 4 (mouse chromosome 5) (3, 4), and they derive from a common ancestor shared with other enamel, milk, and saliva calcium-binding proteins, likely secreted protein acidic and rich in cysteine like-1 (SPARCL-1)/Hevin, which is a relative of SPARC/osteonectin (5). Bone sialoprotein (BSP), an early marker of osteoblast differentiation. By using chromatin immunoprecipitation (ChIP) analysis in MC3T3-E1 (clone MC-4) preosteoblast cells, RUNX2 was shown to . It is a phosphorylated glycoprotein with an approximate molecular weight of 70 kDa. Chlorpromazine (10 g/ml) decreased luciferase activity of the construct (pLUC3;-116 to +60 of the rat BSP . However, the . Dual non-viral gene delivery from microparticles within 3D high-density stem cell constructs for enhanced bone tissue engineering. Bone integrin-binding sialoprotein, usually referred to as bone sialoprotein (BSP), is the second major sialoprotein of bone [7]. The bone sialoprotein (Bsp) gene provides an excellent model for studying mechanisms controlling osteoblast-specific gene expression. BSP is a significant component of the bone extracellular matrix and has been suggested to constitute approximately 8% of all non-collagenous proteins found in bone and cementum. It binds to calcium and hydroxyapatite, cells, and collagens. The deduced 317-amino acid protein has a calculated molecular mass of about 35 kD. Alsberg E Biomaterials 161. Bone sialoprotein (BSP) is the structural glycoprotein of the bone matrix that defines the initial process of . Bone sialoprotein (BSP) belongs to the "small integrin-binding ligand N-linked glycoprotein" (SIBLING) family, whose members interact with bone cells and bone mineral. Bone sialoprotein (BSP) is a major noncollagenous protein of the mineralized bone extracellular matrix that has been implicated in the nucleation of hydroxyapatite. The presence of both bone sialoprotein-binding protein gene and collagen adhesin gene as a typical virulence trait of the major epidemic cluster in isolates from orthopedic implant infections. These studies pave the way for novel gene discovery and biomarker validation for cancer progression. . The protein encoded by this gene is a major structural protein of the bone matrix. BSP is a highly glycosylated and sulphated phosphoprotein that is found almost exclusively in mineralized connective tissues. Bone sialoprotein (BSP) is a mineralized tissuespecific protein that appears to function in the initial mineralization of bone. Invitrogen Anti-Bone SialoProtein Polyclonal, Catalog # PA5-79425. Recent studies have shown that BSP is also expressed by osteotropic cancers suggesting BSP might play a role in the pathogenesis of bone metastases. Dental pulp tissue represents a source of mesenchymal stem cells that have a strong differentiation potential towards the osteogenic lineage. It constitutes approximately 12% of the non-collagenous protein of human bone. Gene transfection into cells is an established technique to induce cellular functions. Conclusion: Bone sialoprotein protects cells from complement-mediated cellular lysis, activates matrix metalloproteinase 2 and has an angiogenic capacity. H ydrogels are a logical choice for nonload-bearing bone tissue-engineering applications, as they exhibit excellent biocompatibility and cause minimal inflammatory responses and tissue damage. bone sialoprotein, Bsp, BSP, Bsp2 Feature Type. Bone Sialoprotein Gene Transfer to Periodontal Ligament Cells Volume 77 Number 2 170 the BSP-decorin chimeric protein could be capable of inducingthemineralizationofcollageninvivo.28Based on this information, BSP is likely to be involved with early mineral deposition. Bone sialoprotein is an acidic glycoprotein of approximately 70 kD that undergoes extensive posttranslational modifications. The only extraskeletal site of its synthesis is the . We have isolated and partially sequenced the human bone sialoprotein gene (IBSP). Whereas physical forces may play an important role in the regulation of bone cell function, little is known about how cells are able to sense mechanical loads. The purpose of this review is to summarize recent functional and structural findings regarding non-collagenous matrix proteins in bone and teeth, to compare gene locations for bone and tooth matrix. (1990) cloned the human ortholog from a cultured bone cell cDNA library. Bone sialoprotein (BSP) is a component of mineralized tissues such as bone, dentin, cementum and calcified cartilage. Several studies have shown that EMD regulates the proliferation, migration, adhesion, gene expression, and cytokine production of (pre-)osteoblasts, periodontal fibroblasts, and gingival fibroblasts. The results showed that MG63 cells cultured on A-SrHANF had higher osteogenesis-related gene expression than those cultured on A-HANF. Bone sialoprotein is involved in migration of bone marrow stromal cells through Matrigel and collagen barriers. Bone sialoprotein and its transcriptional regulatory mechanism. IBSP has been sublocalized by in situ hybridization to chromosome 4q28-q31 and is composed of six small exons (51 to 159 bp) and 1 large exon (2.6 kb). Integrin-binding sialoprotein is an approximately 70 kDa acidic glycoprotein encoded by the human IBSP gene 29. 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